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知花博治

職種 准教授
所属プロジェクト カンジダフェノームプロジェクト
研究部門 真菌症研究部門
分野 病原機能分野
TEL 043-226-2792
FAX 043-226-2792
E-mail chibana[at]faculty.chiba-u.jp
プロフィール写真

専門分野

分子生物学、医真菌学

研究テーマ

カンジダ・グラブラータの病原機能解析と抗真菌薬標的の探索

国立大学共同利用・共同研究拠点協議会 YouTube公式チャンネル 知の拠点【すぐわかアカデミア。】「すぐにわかるもっとも身近な真核生物、カビと酵母 ~あなたの体はシェアハウス~」
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所属学協会

日本医真菌学会、日本細菌学会、日本分子生物学会、日本微生物ゲノム学会、American Society for Microbibology

主な発表論文、著書

    Researchmap

    1. Pedro P, Monica G, Takahashi-Nakaguchi A, Chibana H, Miguel C.T: Multiple genome analysis of Candida glabrata clinical isolates renders new insights into genetic diversity and drug resistance determinants. Microbial Cell, in press.
    2. Konuma R, Watanabe M, Irikura D, Sugita-Konishi Y, Yamazaki A, Yanagi U, Doha Y, Kobayashi N, Chibana H, Onami J, Kamata Y: Polymorphism of Aspergillus Fumigatus Major Allergen Genes Associating with Their Isolated Sites Affects Their IgE Epitope Structures. Fungal Genomics & Biology, 12. 4: 1000195, 2022.
    3. Okamoto M, Takahashi-Nakaguchi A, Tejima K, Sasamoto K, Yamaguchi M, Aoyama T, Nagi M, Tanabe K, Miyazaki Y, Nakayama H, Sasakawa C, Kajiwara S, Brown AJP, Teixeira MC, Chibana H: Erg25 Controls Host-Cholesterol Uptake Mediated by Aus1p-Associated Sterol-Rich Membrane Domains in Candida glabrata. Front Cell Dev Biol, 24;10:820675, 2022.
    4. Pais P, Galocha M, California R, Viana R, Ola M, Okamoto M, Chibana H, Butler G, Teixeira MC: Characterization of the Candida glabrata Transcription Factor CgMar1: Role in Azole Susceptibility. J Fungi (Basel), 7;8(1):61.10, 2022.
    5. Yamaguchi M, Takahashi-Nakaguchi A, Uematsu K, Yamada H, Sato-Okamoto M, Chibana H: Ultrastructural examination of mouse glomerular capillary loop by sandwich freezing and freeze-substitution, Microscopy (Oxf), 00010.R2, 2022.
    6. Cavalheiro M, Pereira D, Formosa-Dague C, Leitao C, Pais P, Ndlovu E, Viana R, Pimenta AI, Santos R, Takahashi-Nakaguchi A, Okamoto M, Ola M, Chibana H, Fialho AM, Butler G, Dague E, Teixeira MC: From the first touch to biofilm establishment by the human pathogen Candida glabrata: a genome-wide to nanoscale view. Commun Biol, 4(1) 886-886, 2021.
    7. Yao Huang, Keisuke Fujii, Xinyue Chen, Shun Iwatani, Hiroji Chibana, Soichi Kojima, Susumu Kajiwara: Erratum to: Fungal NOX is an essential factor for induction of TG2 in human hepatocytes. Medical mycology, 59(5) 514-514, 2021.
    8. Pedro P, Catarina C, Monica G, Diana P, Takahashi-Nakaguchi A, Chibana H, Arsenio F, Miguel T: Role of CgTpo4 in polyamine and antimicrobial peptide resistance: determining virulence in Candida glabrata. International Journal of Molecular Sciences, 2021.
    9. Xinyue C, Iwatani S, Kitamoto T, Chibana H, Kajiwara S: The lack of SNARE protein homologue Syn8 influences biofilm formation of Candida glabrata. Frontiers in Cell and Developmental Biology, 2021.
    10. Yamaguchi M, Takahashi-Nakaguchi A, Uematsu K, Taguchi M, Sato-Okamoto M, Chibana H: Rapid freezing using Sandwich Freezing Device for good ultrastructural preservation of 4 viruses, yeast, and animal tissues in electron microscopy. Journal of Visualized Experiments, 2021.
    11. Galkina V, Okamoto M, Chibana H, Knorre A, Kajiwara S: Deletion of CDR1 reveals redox regulation of pleiotropic drug resistance in Candida glabrata. Biochimie, 170:49-56, 2020.
    12. Santos R, Cavalheiro M, Costa C, Takahashi-Nakaguchi A, Okamoto M, Chibana H, Teixeira MC; Screening the Drug:H+ Antiporter Family for a Role in Biofilm Formation in Candida glabrata. Front Cell Infect Microbiol, 4;10:29, 2020.
    13. Takahashi-Nakaguchi A, Shishido E, Yahara M, Urayama S, Ninomiya A, Chiba Y, Sakai K, Hagiwara D, Chibana H, Moriyama H, Gonoi T: Phenotypic and Molecular Biological Analysis of Polymycovirus AfuPmV-1M from Aspergillus fumigatus: Reduced Fungal Virulence in a Mouse Infection Model. Frontiers in Microbiology, 11:607795, 2020.
    14. Takahashi-Nakaguchi A, Shishido E, Yahara M, Urayama S, Sakai K, Chibana H, Kamei K, Moriyama H, Gonoi T :Analysis of an intrinsic mycovirus associated with reduced virulence of the human pathogenic fungus Aspergillus fumigatus. Frontiers in Microbiology, 17;10:3045, 2020.
    15. Yamaguchi M, Yamada H, Chibana H: Deep-sea bacteria harboring bacterial endosymbionts in a cytoplasm? 3D electron microscopy by serial ultrathin sectioning of freeze-substituted specimen. Cytologia, 85(3) 209-211, 2020.
    16. Huang Y, Fujii K, Chen X, Iwatani S, Chibana H, Kojima S, Kajiwara S: Fungal NOX is an essential factor for induction of TG2 in human hepatocytes. Med Mycol, 2019. [Epub ahead of print]
    17. Horinouchi Y, Yamaguchi M, Chibana H, Togashi T: Nuclear behavior and roles indicate that Codiolum phase is a sporophyte in Monostroma angicava (Ulotrichales, Ulvophyceae). J Phycology. 55: 534-542, 2019.
    18. Ikezaki S, Cho T, Nagao J, Tasaki S, Yamaguchi M, Arita-Morioka K, Yasumatsu K, Chibana H, Ikebe T, Tanaka Y: Mild heat stress affects on the cell wall structure in Candida albicans biofilm. Med Mycol J 60: 29-37, 2019.
    19. Onami JI, Kobayashi N, Watanabe M, Yamada O, Mizutani O, Yokoyama K, Haruo T, Chibana H, Kamata Y; An updated data portal for fungal allergens with curated information. Bioinformation, 11;15(11):820-823, 2019.
    20. Shiratori R, Furuichi K, Yamaguchi M, Miyazaki N, Aoki H, Chibana H, Ito K, Aoki S; Glycolytic suppression dramatically changes the intracellular metabolic profile of multiple cancer cell lines in a mitochondrial metabolism-dependent manner. Sci Rep, 10;9(1):18699, 2019.
    21. Stepanova A.A., VasilyevaN.V., Yamaguchi M., Chibana H. , Chilina G.A. , Bogomolova T.S. The peculiarity of Aspergillus tubigensis micromorphology: scanning electron microscopy. Problems in Medical Mycology, 21: 23-26, 2019.
    22. Widiasih T, Chen X, Iwatani S, Chibana H, Kajiwara S: Role of major facilitator superfamily transporter Qdr2p in biofilm formation by Candida glabrata. Mycoses, 62(12):1154-1163, 2019.
    23. Stepanova A, Vasilyeva N, Yamaguchi M, Chibana H, Chilina G, Bosak A: Cytological investigations of cell components migration dynamics during budding in the yeast Cryptococcus neoformans. Problemy medicinskoj mikologii, in press.
    24. Cavalheiro M, Costa C, Silva-Dias A, Miranda I, Wang C, Pais P, Pinto S, Mil-Homens D, Sato-Okamoto M, Takahashi-Nakaguchi A, Silva R, Mira N, Fialho A, Chibana H, Rodrigues A, Butler G, Teixeira M: A Transcriptomics Approach To Unveiling the Mechanisms of In Vitro Evolution towards Fluconazole Resistance of a Candida glabrata Clinical Isolate. Antimicrobial Agents Chemother, 63(1), 2018.
    25. Hirao T, Yamaguchi M, Kikuya M, Chibana H, Ito K, Aoki S: Altered intracellular signaling by imatinib increases the anti-cancer effects of tyrosine kinase inhibitors in CML cells. Cancer Science.109(1):121-131, 2018.
    26. Stepanova A, Vasilyeva N, Yamaguchi M, Chibana H, Bosak, Filippova L: Ultrastructural patterns of interactions between murine lung macrophages and yeast cells of Cryptococcus neoformans strains with different virulence. Med Mycol J, 59(1)E1-E6, 2018.
    27. Takahashi-Nakaguchi A, Sakai K, Takahashi H, Hagiwara D, Toyotome T, Chibana H, Watanabe A, Yaguchi T, Yamaguchi M, Kamei K, Gonoi T.Aspergillus fumigatus adhesion factors in dormant conidia revealed through comparative phenotypic and transcriptomic analyses.Cell Microbiol.20(3):e12802,2018.
    28. Tanaka Y, Sasaki M, Ito F, Aoyama T, Sato-Okamoto M, Takahashi-Nakaguchi A, Chibana H, Shibata N: Cooperation between ER stress and calcineurin signaling contributes to the maintenance of cell wall integrity in Candida glabrata. Fungal Biol, 122(1):19-33, 2018
    29. Yamada H, Yamaguchi M, Igarashi Y, Chikamatsu K, Aono A, Murase Y, Morishige Y, Takaki A, Chibana H, Mitarai S: Mycolicibacterium smegmatis, Basonym Mycobacterium smegmatis, Expresses Morphological Phenotypes Much More Similar to Escherichia coli Than Mycobacterium tuberculosis in Quantitative Structome Analysis and CryoTEM Examination. Front Microbiol, 11;9:1992, 2018.
    30. Yamaguchi M, Chibana H : A Method for Obtaining Serial Ultrathin Sections of Microorganisms in Transmission Electron Microscopy.J Vis Exp.(131):e56235,2018.
    31. Salazar S, Wang C, Munsterkotter M, Okamoto M, Takahashi-Nakaguchi A, Chibana H, Lopes M, Guldener U, Mira N: Comparative genomic and transcriptomic analyses unveil novel features of azole resistance and adaptation to the human host in Candida glabrata. FEMS Yeast Res. 18(1), 2018.
    32. Aoki S, Morita M, Hirao T, Yamaguchi M, Shiratori R, Kikuya M, Chibana H, Ito K: Shift in energy metabolism caused by glucocorticoids enhances the effect of cytotoxic anti-cancer drugs against acute lymphoblastic leukemia cells. Oncotarget Journal.8(55):94271-94285, 2017.
    33. Bernardo RT, Cunha DV, Wang C, Pereira L, Silva S, Salazar SB, Schröder MS, Okamoto M, Takahashi-Nakaguchi A, Chibana H, Aoyama T, Sá-Correia I, Azeredo J, Butler G, Mira NP. The CgHaa1-Regulon Mediates Response and Tolerance to Acetic Acid Stress in the Human Pathogen Candida glabrata. G3 (Bethesda). 5;7(1):1-18, 2017.
    34. Yamada H, Yamaguchi M, Shimizu K, Murayama S, Mitarai S, Sasakawa C, Chibana H, Structome Analysis of Escherichia coli cells by serial ultra thin sectioning reveals the precise cell profiles and the ribosome density. Microscopy (Oxf), 66:283-294, 2017.
    35. Yamaguchi M, Takahashi-Nakaguchi A, Aida Y, Sato-Okamoto M, Chibana H: Convenient method for better preservation of fine structures of cultured macrophages and engulfed yeast cells by freeze-substitution fixation. Microscopy (Oxf) . 1;66(3):209-211, 2017.
    36. Romão D, Cavalheiro M, Mil-Homens D, Santos R, Pais P, Costa C, Takahashi-Nakaguchi A, M Fialho A, Chibana H, Teixeira MC: A new determinant of Candida glabrata virulence: the acetate exporter CgDtr1. Front Cell Infect Microbiol. 7:473, 2017.
    37. Shrestha R, Shrestha R, Qin X, Kuo T, Oshima Y, Iwatani S, Teraoka R, Fujii K, Hara M, Li M, Takahashi-Nakaguchi A, Chibana H, Lu J, Cai M, Kajiwara S, Kojima S: Fungus-derived hydroxyl radicals kill hepatic cells by enhancing nuclear transglutaminase. Scientific Reports, 2017 Jul 6;7(1):4746
    38. Stepanova A, Vasilyeva V, Yamaguchi M, Chibana H, Bosak A: Ultrastructural investigations of early stages of transformation of yeast from hyphal in Cryptococcus neoformans var. grubii in vivo. Problems in Medical Mycology 19 (No. 2): 19-24, 2017.
    39. Hirayasu K, Saito F, Suenaga T, Shida K, Arase N, Oikawa K, Yamaoka T, Murota H, Chibana H, Nakagawa I, Kubori T, Nagai H, Nakamaru Y, Katayama I, Colonna M, and Arase H. Microbially cleaved immunoglobulins are sensed by the innate immune receptor LILRA2. Nature Microbiology, 1(6):16054, 2016.
    40. Pais P, Costa C, Pires C, Shimizu K, Chibana H, Teixeira MC. Membrane proteome-wide response to the antifungal drug clotrimazole in Candida glabrata: role of the transcription factor CgPdr1 and the Drug:H+ Antiporters CgTpo1_1 and CgTpo1_2. Mol Cell Proteomics ; 15(1):57-72, 2016.
    41. Pais P, Pires C, Costa C, Okamoto M, Chibana H, Teixeira MC. Membrane proteomics analysis of the Candida glabrata response to 5-flucytosine: unveiling the role and regulation of the drug efflux transporters CgFlr1 and CgFlr2. Frontiers in Microbiology, 7:2045, 2016.
    42. Tanaka Y, Sasaki M, Ito F, Aoyama T, Sato-Okamoto M, Takahashi-Nakaguchi A,Chibana H, Shibata N. KRE5 Suppression Induces Cell Wall Stress and Alternative ER Stress Response Required for Maintaining Cell Wall Integrity in Candida glabrata. PLoS One. 22;11(8):e0161371, 2016.
    43. Yamaguchi M, Aoyama T, Yamada N, Chibana H. Quantitative measurement of hydrophilicity/hydrophobicity of the plasma-polymerized naphthalene film (Super Support Film) and other support films and grids in electron microscopy. Microscopy(Oxf), 65(5):444-450, 2016.
    44. Yamaguchi M, Yamada H, Higuchi K, Yamamoto Y, Arai S, Murata K, Mori Y, Furukawa H, Uddin MS, Chibana H: High-voltage electron microscopy tomography and structome analysis of unique spiral bacteria from the deep sea. Microscopy(Oxf), 65(4):363-9, 2016.
    45. Costa C, Ponte A, Pais P, Santos R, Cavalheiro M, Yaguchi T, Chibana H, Teixeira MC. New Mechanisms of Flucytosine Resistance in C. glabrata Unveiled by a Chemogenomics Analysis in S. cerevisiae. PLoS One; 12;10(8), e0135110, 2015.
    46. Nakatsukasa K, Nishimura T, Byrne SD, Okamoto M, Takahashi-Nakaguchi A, Chibana H, Okumura F, Kamura T. The Ubiquitin Ligase SCFUcc1 Acts as a Metabolic Switch for the Glyoxylate Cycle. Mol Cell. 59: 22-34. 2015
    47. Stepanova AA, Vasilyeva NV, Yamaguchi M, Chibana H, Bosak IA. The Aspergillus fumigatus penetration through the cells of murine tracheobronchial epithelium cells. Problems in Medical Mycology 17: 45-50, 2015.
    48. Shimizu K, Imanishi Y, Toh-e A, Uno J, Chibana H, Hull CM, Kawamoto S. Functional characterization of PMT2, encoding a protein-O-mannosyltransferase, in the human pathogen Cryptococcus neoformans. Fungal Genet Biol. 69:13-22, 2014.
    49. Aoyama T, Nakayama H, Ueno K, Inukai T, Tanabe K, Nagi M, Bard M, Chibana H. Genome-wide survey of transcriptional initiation in the pathogenic fungus, Candida glabrata. Genes Cells.19(6).478-503, 2014.
    50. Costa C, Nunes J, Henriques A, Mira NP, Nakayama H, Chibana H, Teixeira MC. Candida glabrata drug:H+ antiporter CgTpo3 (ORF CAGL0I10384g): role in azole drug resistance and polyamine homeostasis. J Antimicrob Chemother. 69(7):1767-76, 2014.
    51. Yamaguchi S, Sano A, Hiruma M, Murata M, Kaneshima T, Murata Y, Takahashi H, Takahashi S, Takahashi Y, Chibana H, Touyama H, Ha NT, Nakazato Y, Uehara Y, Hirakawa M, Imura Y, Terashima Y, Kawamoto Y, Takahashi K, Sugiyama K, Hiruma M, Murakami M, Hosokawa A, Uezato H. Mycopathologia. Isolation of dermatophytes and related species from domestic fowl (Gallus gallus domesticus). Mycopathologia. 178:135-43, 2014.
    52. Costa C, Henriques A, Pires C, Nunes J, Ohno M, Chibana H, Sa-Correia I, Teixeira MC. The dual role of candida glabrata drug:H+ antiporter CgAqr1 (ORF CAGL0J09944g) in antifungal drug and acetic acid resistance. Front Microbiol. 26;4:170, 2013.
    53. Costa C, Pires C, Cabrito TR, Renaudin A, Ohno M, Chibana H, Sa-Correia I, Teixeira MC. Candida glabrata drug:H+ antiporter CgQdr2 confers imidazole drug resistance, being activated by transcription factor CgPdr1. Antimicrob Agents Chemother. 57(7):3159-67, 2013.
    54. Nagi M, Tanabe K, Ueno K, Nakayama H, Aoyama T, Chibana H, Yamagoe S, Umeyama T, Oura T, Ohno H, Kajiwara S, Miyazaki Y. The Candida glabrata sterol scavenging mechanism, mediated by the ATP-binding cassette transporter Aus1p, is regulated by iron limitation. Mol Microbiol. 88(2):371-81, 2013.
    55. Niimi K, Woods MA, Maki K, Nakayama H, Hatakenaka K, Chibana H, Ikeda F, Ueno K, Niimi M, Cannon RD, Monk BC. Reconstitution of high-level micafungin resistance detected in a clinical isolate of Candida glabrata identifies functional homozygosity in glucan synthase gene expression. J Antimicrob Chemother. 67(7):1666-76, 2012.
    56. Ueno K, Matsumoto Y, Uno J, Sasamoto K, Sekimizu K, Kinjo Y, Chibana H. Intestinal resident yeast Candida glabrata requires Cyb2p-mediated lactate assimilation to adapt in mouse intestine. PLoS One. 6(9):e24759, 2011.
    57. Chibana H, Magee PT. The enigma of the major repeat sequence of Candida albicans. Future Microbiol. 4(2):171-9, 2009.
    58. Nakayama H, Tanabe K, Bard M, Hodgson W, Wu S, Takemori D, Aoyama T, Kumaraswami NS, Metzler L, Takano Y, Chibana H, Niimi M. The Candida glabrata putative sterol transporter gene CgAUS1 protects cells against azoles in the presence of serum. J Antimicrob Chemother. 60(6):1264-72, 2007.
    59. Ueno K, Uno J, Nakayama H, Sasamoto K, Mikami Y, Chibana H. Development of a highly efficient gene targeting system induced by transient repression of YKU80 expression in Candida glabrata. Eukaryot Cell. 6(7):1239-47, 2007.
    60. van het Hoog M, Rast TJ, Martchenko M, Grindle S, Dignard D, Hogues H, Cuomo C, Berriman M, Scherer S, Magee BB, Whiteway M, Chibana H, Nantel A, Magee PT. Assembly of the Candida albicans genome into sixteen supercontigs aligned on the eight chromosomes. Genome Biol. 8(4):R52, 2007.
    61. Chibana H, Oka N, Nakayama H, Aoyama T, Magee BB, Magee PT, Mikami Y. Sequence finishing and gene mapping for Candida albicans chromosome 7 and syntenic analysis against the Saccharomyces cerevisiae genome. Genetics.170(4):1525-37, 2005.
    62. Jones T, Federspiel NA, Chibana H, Dungan J, Kalman S, Magee BB, Newport G, Thorstenson YR, Agabian N, Magee PT, Davis RW, Scherer S. The diploid genome sequence of Candida albicans. Proc Natl Acad Sci U S A. 11;101(19):7329-34, 2004.
    63. Chibana H, Beckerman JL, Magee PT. Fine-resolution physical mapping of genomic diversity in Candida albicans. Genome Res. 10(12):1865-77, 2000.

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      国立大学共同利用・共同研究拠点協議会 YouTube公式チャンネル 知の拠点【すぐわかアカデミア。】「すぐにわかるもっとも身近な真核生物、カビと酵母 ~あなたの体はシェアハウス~」
      >>動画はこちら